https://www.tsvetanovalab.com/home daily 1.0 2023-05-09 https://images.squarespace-cdn.com/content/v1/5ba94295da50d33a2f4f96aa/1540414752886-2N28LCTOFOBF2MC4O85V/T-lab.png Home https://www.tsvetanovalab.com/contact daily 0.75 2018-09-25 https://www.tsvetanovalab.com/technology daily 0.75 2021-07-09 https://images.squarespace-cdn.com/content/v1/5ba94295da50d33a2f4f96aa/1537889592196-BRF42T0TOK1B2YF2IWF5/bPAC.png Technology Figure 1- Site-specific generation of cAMP in cells. A light-activated adenylyl cyclase is targeted to the plasma membrane (PM), endosomes, or cytosol for local generation of cAMP using light. https://images.squarespace-cdn.com/content/v1/5ba94295da50d33a2f4f96aa/1537889003249-WLXAJWW1JFBCT66CRFWX/CRISPRi.png Technology Figure 2- Screen for regulators of GPCR/cAMP signaling. Cells expressing a fluorescent transcriptional CREB reporter are transduced with a genomic CRISPRi library, treated with drug, and separated by FACS based on ligand effects on reporter expression. Genomic DNA is isolated from each population, and analyzed by deep sequencing to identify regulators. https://www.tsvetanovalab.com/news daily 0.75 2025-10-20 https://www.tsvetanovalab.com/publications daily 0.75 2025-08-28 https://www.tsvetanovalab.com/pharmacology2 daily 0.75 2021-01-11 https://images.squarespace-cdn.com/content/v1/5ba94295da50d33a2f4f96aa/1537891888023-XXBR0D0IZWECLV4JWZGH/LigandDiscrimination.png Pharmacology Figure- Ligand discrimination. Different ligands binding the same GPCR vary in their endocytic efficacy. Does ligand-induced internalization underlie drug specificity? Genes encoding receptors and other signaling components are polymorphic in the human population (red star denotes a SNP in the GPCR gene). How do variants affect GPCR trafficking and ligand-dependent signaling? https://www.tsvetanovalab.com/physiology daily 0.75 2023-09-13 https://images.squarespace-cdn.com/content/v1/5ba94295da50d33a2f4f96aa/1610405443339-70PZEC8AFOOCEIGX538C/iNeurons_nanobody.png Physiology Figure. Active B2-AR on endosomes of human iPSC-derived neurons. A nanobody bionsensor (Nb80) detects active flag-tagged receptor (red). https://www.tsvetanovalab.com/positions daily 0.75 2025-09-04 https://www.tsvetanovalab.com/research daily 0.75 2020-09-29 https://images.squarespace-cdn.com/content/v1/5ba94295da50d33a2f4f96aa/1537884968882-60H2SVP0IFJ0VATYN4AF/Physiology.png RESEARCH https://images.squarespace-cdn.com/content/v1/5ba94295da50d33a2f4f96aa/1537885576303-EBM6VPGNEIJRJ3A9MHQQ/Pharmacology.png RESEARCH https://images.squarespace-cdn.com/content/v1/5ba94295da50d33a2f4f96aa/1537885469468-29OOX7CHTILN4LHQCSE2/Regulation.png RESEARCH https://images.squarespace-cdn.com/content/v1/5ba94295da50d33a2f4f96aa/1537884919706-TZ31O1L25GKEVXZ45JWV/Technology2.png RESEARCH https://www.tsvetanovalab.com/regulation2 daily 0.75 2025-02-27 https://images.squarespace-cdn.com/content/v1/5ba94295da50d33a2f4f96aa/1537888218679-CLX2E2FSPOBNDADJYK1D/Reg2.png Regulation Figure- Spatial encoding of GPCR/cAMP cellular responses. Left, The classical GPCR model: receptors sense ligands and initiate signal transduction from the cell surface, but are turned “off” once internalized. Right, A revised model based on our data: GPCRs activation and cAMP production from endosomal receptors is more efficiently coupled to the cellular transcriptional response. https://www.tsvetanovalab.com/lab-fun daily 0.75 2025-10-06 https://images.squarespace-cdn.com/content/v1/5ba94295da50d33a2f4f96aa/1583164735072-IB6XSDQQO4A6E1HTMFBG/IMG_5759.jpg LAB FUN Pour, Durham https://images.squarespace-cdn.com/content/v1/5ba94295da50d33a2f4f96aa/1558894392317-PHREIH9XQW4P4ZUFAXOW/IMG_4935.jpg LAB FUN https://images.squarespace-cdn.com/content/v1/5ba94295da50d33a2f4f96aa/4653e6f4-2c8b-413b-8e30-1f0538994ec9/VelverHippo_1.jpg LAB FUN - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/5ba94295da50d33a2f4f96aa/1572525711809-E2Q40NVIHLIR0WQCBRBB/Dashi_20302019.jpg LAB FUN Dashi, Durham https://images.squarespace-cdn.com/content/v1/5ba94295da50d33a2f4f96aa/1572609328688-XMOPX2XM20JISXTWGB00/IMG_5482.JPG LAB FUN GOT-themed Halloween 2019 https://images.squarespace-cdn.com/content/v1/5ba94295da50d33a2f4f96aa/95e8bcab-22df-4c7e-9bd8-5ca250355821/Halloween.jpg LAB FUN - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/5ba94295da50d33a2f4f96aa/1620662455128-Z710H50434JA4B5ZZ8FB/thumbnail_IMG_6467.jpg LAB FUN - Make it stand out May 2021: The Tlab & family/friends got to properly mark all of our achievements (paper, grants, PhD admissions) from the past year. https://images.squarespace-cdn.com/content/v1/5ba94295da50d33a2f4f96aa/1631276181150-JE5J71Q2QA63TXOXCOQB/WaDu.jpg LAB FUN - Make it stand out Washington Duke Inn, 09/09/2021 https://images.squarespace-cdn.com/content/v1/5ba94295da50d33a2f4f96aa/1623345487910-1034BWMJIZWGB8LQZGD3/Mattprelim01.jpg LAB FUN - Make it stand out Brownies courtesy of Nisa! https://images.squarespace-cdn.com/content/v1/5ba94295da50d33a2f4f96aa/1558894461947-4US0MOCMTZE2V5UDL6I5/IMG_4939.jpg LAB FUN Urban Axes Durham, 05/23/2019 https://images.squarespace-cdn.com/content/v1/5ba94295da50d33a2f4f96aa/c15dca9d-fd96-44ab-a0bd-d9da9d6dfdc2/thumbnail_IMG_7986.jpg LAB FUN - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/5ba94295da50d33a2f4f96aa/296f2460-e0ed-4d3c-b525-e88e87edc9a9/thumbnail_IMG_8334.jpg LAB FUN - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/5ba94295da50d33a2f4f96aa/4d5eddf1-6536-46a2-9a4f-078990cc5593/thumbnail_IMG_8216.jpg LAB FUN - Make it stand out Our first award- Katherine’s pumpkin won ‘PCB Best Carved Pumpkin’ https://images.squarespace-cdn.com/content/v1/5ba94295da50d33a2f4f96aa/1623345501960-RZTJDS9RLX8E8G9BAZGG/Mattprelim02.jpg LAB FUN - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/5ba94295da50d33a2f4f96aa/81e4a308-6390-4f9d-a442-4063230083fc/Picture1.jpg LAB FUN - Make it stand out Durham Bulls game, 09/14/25 https://images.squarespace-cdn.com/content/v1/5ba94295da50d33a2f4f96aa/edbbbb24-01e0-4a99-b126-8787b7e18d02/NC_Escape_1.jpeg LAB FUN - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/5ba94295da50d33a2f4f96aa/c3bd7563-7fa4-4e49-92c7-a8f08c279930/Picture1.png LAB FUN - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/5ba94295da50d33a2f4f96aa/43f41633-440c-40ae-b215-0312593c0461/IMG_8591.jpg LAB FUN - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/5ba94295da50d33a2f4f96aa/1583164698856-QVXD76PBS65MFWKD6ND8/IMG_5757.jpg LAB FUN Bull City Escape, Durham 2/29/2020 https://images.squarespace-cdn.com/content/v1/5ba94295da50d33a2f4f96aa/e0219d87-4028-4606-a0eb-e61c19e6fc0c/Picture1.png LAB FUN - Make it stand out The lab had an excellent time at the annual Duke Pharmacology & Cancer Bio retreat (Wrightsville beach)! Katherine gave a great talk, and she and Matt each won a Fitzgerald award to recognize success in obtaining a fellowship and an impactful publication, respectively. https://images.squarespace-cdn.com/content/v1/5ba94295da50d33a2f4f96aa/821c27ca-e17d-4f3d-8f53-4f8f88d67250/Tlab2023.jpg LAB FUN - Make it stand out We have a lot to celebrate during this and the coming months. Let’s start with congratulating our awesome technician Angelica on her admission to graduate school, and officially welcome Meagan to the lab. https://images.squarespace-cdn.com/content/v1/5ba94295da50d33a2f4f96aa/8f9ce36a-f2c5-44d0-b5cd-446c0c60ce96/Picture1.png LAB FUN - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/5ba94295da50d33a2f4f96aa/4f1e125f-438f-46bd-b46d-4408f56f2a4f/Picture1.png LAB FUN - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/5ba94295da50d33a2f4f96aa/49aa11ee-986a-4ebd-92a9-1c9f8914db44/Picture1.png LAB FUN - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/5ba94295da50d33a2f4f96aa/1620662486270-99QWAIRP0TS2R5O0G7AN/thumbnail_IMG_6471.jpg LAB FUN - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/5ba94295da50d33a2f4f96aa/1558894379432-O2K2ZEOW34RDXV3O4Q1I/IMG_4923.jpg LAB FUN https://images.squarespace-cdn.com/content/v1/5ba94295da50d33a2f4f96aa/3868382e-9b18-4589-916f-90ebc287c181/Picture2.png LAB FUN - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/5ba94295da50d33a2f4f96aa/782fb250-4835-4419-bcae-ee2476b245dc/IMG_8015.jpg LAB FUN - Make it stand out Urban axes, Durham https://images.squarespace-cdn.com/content/v1/5ba94295da50d33a2f4f96aa/1590888130303-RX1FJN5RJ9KM8XYKRZXW/Screen%2BShot%2B2020-05-30%2Bat%2B8.13.02%2BPM.jpg LAB FUN Zoom Happy Hour to celebrate Blair and Nisa passing their prelims #socialdistance, 5/30/2020 https://images.squarespace-cdn.com/content/v1/5ba94295da50d33a2f4f96aa/03302aa0-d7e1-4e0a-a4a0-6e77f2b2ee9b/Picture1.png LAB FUN - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/5ba94295da50d33a2f4f96aa/e06eb08a-25da-4edc-8aaa-b8b3fa16c99d/Picture1.jpg LAB FUN - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/5ba94295da50d33a2f4f96aa/ab4b84cf-6a4f-4e97-bee4-a8e59a8a5172/BW.png LAB FUN - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/5ba94295da50d33a2f4f96aa/2aa39b13-7d77-4812-98d2-2ee2bd74960a/thumbnail_IMG_7202.jpg LAB FUN - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/5ba94295da50d33a2f4f96aa/3eb31322-b86e-4d7f-b6c7-588df7e3693d/Picture1.png LAB FUN - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/5ba94295da50d33a2f4f96aa/667fa6c4-9dd8-4ac3-a3cd-a2d712044556/Picture1.jpg LAB FUN - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/5ba94295da50d33a2f4f96aa/1558894404088-BZIKX1KBPR0IKU3LCEXU/IMG_4931.jpg LAB FUN Urban Axes Durham, 05/23/2019 https://images.squarespace-cdn.com/content/v1/5ba94295da50d33a2f4f96aa/6284f29e-9835-4e03-8c37-b256980665b1/IMG_8599.JPG LAB FUN - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://www.tsvetanovalab.com/pagemk daily 0.75 2025-03-10 https://www.tsvetanovalab.com/pagent daily 0.75 2025-05-07 https://www.tsvetanovalab.com/technician-ad daily 0.75 2025-09-04 https://www.tsvetanovalab.com/pagekh daily 0.75 2025-08-28 https://www.tsvetanovalab.com/alumni daily 0.75 2025-06-30 https://www.tsvetanovalab.com/pagems daily 0.75 2025-05-01 https://www.tsvetanovalab.com/pon daily 0.75 2025-11-25 https://www.tsvetanovalab.com/pagejh daily 0.75 2025-05-01 https://www.tsvetanovalab.com/new-page-2 daily 0.75 2025-10-20 https://images.squarespace-cdn.com/content/v1/5ba94295da50d33a2f4f96aa/3395a497-53b8-47d9-b8c4-585e2d245ef4/TsvetanovaNG.png Lab members Nina Tsvetanova, Ph.D. Principal Investigator (PCB) https://images.squarespace-cdn.com/content/v1/5ba94295da50d33a2f4f96aa/de7301c2-e704-46e0-bc3c-da27116fbcd7/KatherineH.jpg Lab members Katherine Hall Graduate Student (Pharm) https://images.squarespace-cdn.com/content/v1/5ba94295da50d33a2f4f96aa/1ab4398d-c5e0-4815-bd20-755b6e4d3f87/IMG_0778.jpg Lab members - Make it stand out Jada Barnes, M.S. Research Technician https://images.squarespace-cdn.com/content/v1/5ba94295da50d33a2f4f96aa/083a93e0-e1fd-470c-9104-79dba89028ab/Malarie_S.jpeg Lab members Malarie Schexnider Graduate Student (UPGG) https://images.squarespace-cdn.com/content/v1/5ba94295da50d33a2f4f96aa/f0a86dc0-53f3-4115-b699-d31e73afd149/Tsvetanova_v2.png Lab members - Make it stand out Art credit: Katherine Hall https://images.squarespace-cdn.com/content/v1/5ba94295da50d33a2f4f96aa/c388f14f-3aa4-4ffe-a363-2af4724746aa/IMG_4572.jpg Lab members - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/5ba94295da50d33a2f4f96aa/4b772a77-1447-42a1-b42d-3ac704d49112/JohnH.png Lab members John Hanson Graduate Student (Pharm) https://images.squarespace-cdn.com/content/v1/5ba94295da50d33a2f4f96aa/96d0105c-b2bd-4186-92a3-61b0dde21597/MarcoKurdoglu.jpeg Lab members - Make it stand out Marco Kurdoglu Undergraduate Student https://images.squarespace-cdn.com/content/v1/5ba94295da50d33a2f4f96aa/185dbb52-f690-4155-bd85-ab1af4457913/PeterON.jpg Lab members Peter Opara-Nadi Graduate Student (Pharm)